Effect of freeze-dried bone xenograft and platelet-rich fibrin compound on osteogenesis and osseointegration of alveolar bone defects

doi:10.3969/j.issn.2095-4344.2014.52.003

Abstract

Abstract:

BACKGROUND: Freeze-dried bone xenograft has good osteoconductive ability and low antigenicity, but exhibits relatively poor osteoinductive ability. However, autologous platelet rich fibrin possesses good osteoinductive ability.

OBJECTIVE: To observe the effect of freeze-dried bone xenograft/platelet rich fibrin compound on bone defect repairing and osseointegration around the implant.

METHODS: Twelve normal New Zealand white rabbits were used to prepare the bone defect models at the edentulous area of the bilateral mandible. One titanium screw was implanted in the mesial and distal bone wall of bilateral bone defects respectively. The defects in the right side as experimental group were filled with freeze-dried bone xenograft/platelet rich fibrin compound, while the left defects as control group were inserted only by the freeze-dried bone xenograft. The intact mandible specimens were harvested at 4, 8, 12 weeks after implantation separately, and tested by gross observation, torque test and histological observation.

RESULTS AND CONCLUSION: At 4 weeks after implantation, the experimental group had new vessel formation and a large amount of osteoblasts that were arranged tightly within the monolayer on the surface of newborn bone trabecula; the control group was found to have inflammatory cell infiltration, an increase in fibrous tissue and sparse new vessels, but the number of osteoblasts and new bones was less than that of the experimental group. At 8 weeks after implantation, a great amount of new vessels were seen in the experimental group, and dense bone trabeculae exhibited a mesh-like interconnection, part of which were gradually fused in an island-like shape; in the control group, the number of new vessels was less than that of the experimental group, and the bone trabeculae were slim and scattered. At 12 weeks after implantation, the newborn bone tissue was mature and highly calcified bone trabeculae were seen in the experimental group with formation of lamellar bone; in the control group, the bone trabeculae were integrated into the film that tended to be mature, but the maturity was less than that of the experimental group. Implant-bone bonding strength was higher in the experimental group than the control group at different time points (P < 0.05). These findings indicate that platelet rich fibrin/freeze-dried bone xenograft compound displays good bone repair and osseointegration abilities.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, tissue engineering, compomers

CLC Number:

R318

Li Bing-ting, Jia Ying-zhen, Liu Zhi-fang, Song Yuan, Hou Xiao-wei. Effect of freeze-dried bone xenograft and platelet-rich fibrin compound on osteogenesis and osseointegration of alveolar bone defects [J]. Chinese Journal of Tissue Engineering Research, 2014, 18(52): 8376-8381.

Figures/Tables 3

2.1 动物观察结果 12只实验动物术后当天活动减少，精神﹑饮食受影响，伤口稍肿胀，未见渗出；术后2 d活动与进食恢复术前状态，伤口处干燥，未见感染；术后1周伤口一期愈合。 2.2 标本大体形态观察结果术后4周，螺纹钉均无松动脱落，软组织覆盖；实验组缺损区凹陷，异种冻干骨部分吸收，中央处可见一层薄膜样组织；对照组缺损区边缘较锐利，凹陷更为明显。术后8周，钛螺纹钉均无松动脱落；实验组缺损区异种冻干骨大部分被吸收，被骨痂所替代，骨质质地较硬；对照组缺损区凹陷略深，部分异种冻干骨残留，骨痂较薄钛螺纹钉缺损侧覆盖的骨组织少。术后12周，钛螺纹钉均无松动脱落，被骨组织覆盖；实验组缺损区与周围平齐，与周围组织无明显界限；对照组缺损中央处稍有凹陷。 2.3 扭矩测试结果术后4，8，12周，实验组扭矩峰值均高于对照组(P < 0.05)，见表1。 2.4 组织学观察结果术后4周：实验组缺损区可见新生血管，成骨活跃，大量骨细胞被埋入新形成的骨基质中，形成骨陷窝，成骨细胞数量多，呈单层紧密排列在新生骨小梁的表面；对照组可见炎性细胞浸润，纤维组织增多，新生血管稀疏，成骨细胞及新生骨数量较实验组少，骨小梁纤细，散在(图1A，B)。术后8周：实验组缺损区见大量新生血管，管腔内充满血液，骨小梁密集，呈网状连接，部分已逐渐融合成岛状；对照组新生血管较实验组少，骨小梁纤细，散乱，岛状新生骨较少，成熟度低(图1C，D)。术后12周：实验组新生骨组织成熟，骨小梁钙化程度高，可见形成板状新骨；对照组骨小梁融合成片，趋向成熟，但成熟度不及实验组(图1E，F)。新生骨定量分析：术后4，8，12周，实验组新生骨面积百分比均高于对照组(P < 0.05)，见表2。"

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